Temporal Dynamics of Pathogens and Antibiotic Resistance in Raw and Treated Stormwater

Stormwater is considered as an alternative water source for both potable and non-potable
uses. However, stormwater has not been widely used as an alternative water source, most likely
due to a lack of knowledge about the presence and risks associated with pathogens and antibiotic
resistant bacteria present in raw and treated stormwater. The proposed study will analyze
temporal dynamics of various pathogens and antibiotic resistance genes (ARG) in raw and
treated stormwater samples, by using a high-throughput pathogen and ARG quantification tool.
Pathogen and ARG removal efficiencies during stormwater reclamation processes will be also
calculated. In addition, correlations between pathogen/ARG levels and environmental parameters
such as temperature and precipitation will be analyzed. With this analysis, we will be able to
assess the impact of precipitation and other environmental variables on pathogen/ARG
concentrations in stormwater. This study will significantly advance our understandings in (1)
how pathogens and ARGs occur in Minnesota stormwater and (2) how well these contaminants
can be removed during the stormwater reclamation processes. This study will help stormwater
practitioners and managers better manage their stormwater reuse systems, and will contribute to
the establishment of safe and efficient reuse of Minnesota’s stormwater.

Project Final Report (.pdf)

2019 Midproject Presentation (.pdf)

Project Staff: 

Satoshi Ishii, Assistant Professor, Department of Soil, Water, and Climate, University of
Minnesota, St. Paul, MN (Email: ishi0040@umn.edu; Phone: 612-624-7902)
Anita Anderson, Principal Engineer Supervisor, Drinking Water Protection Section,
Minnesota Department of Health
Nancy Rice, Research Scientist, Environmental Surveillance and Assessment Section,
Minnesota Department of Health

Project Duration: 

January 1, 2019- December 31, 2019